A Grand Rapids native, Main Lnoper attended Grand Valley State University, graduating with a Bachelor's of Fine Arts with a concentration in illustration. She went on to graduate school at Johns Hopkins University School of Medicine, and received a Master's degree in Medical Illustrations. Since her graduation in 2006, Main Knoper has been a medical illustration for the New England Journal of Medicine. In addition to creating visual solutions for the print journal, she develops interactive modules, coding in ActionScript for the web-based journal. She also maintains a small independent medical illustration studio, Kimberly Main Studios.